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Exome capture sequencing of extracted DNA was done by IDTxGen lockdown human panel (Admera Health, LLC South Plainfield, NJ).
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Captured nucleotides were subjected to 150–base pair paired-end sequencing on an Illumina HiSeq platform (Admera Health, South Plainfield, NJ).
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Exome sequencing was performed by Admera Health.
HIF-2 Complex Dissociation, Target Inhibition, and Acquired Resistance with PT2385, a First-in-Class HIF-2 Inhibitor, in Patients with Clear Cell Renal Cell Carcinoma
Whole-exome sequencing (WES) was performed by Admera Health.
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For WES, tumor DNA samples from 16 formalin-fixed, paraffin-embedded (FFPE) tumors and germline DNA samples from four buffy coats were extracted and sequenced at Admera Health.
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Whole-exome sequencing (WES) was performed with an Illumina HiSeq platform (Admera Health, New Jersey), aiming for a 90% coverage of the IDT Xgen library, human genome version 38 (hg38, December 2013)
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Exome capture sequencing of extracted DNA was done by IDTxGen lockdown human panel (Admera Health, LLC South Plainfield, NJ).
Alexa M Barber and others, Whole-genome sequencing to investigate a possible genetic basis of perosomus elumbis in a calf resulting from a consanguineous mating, Translational Animal Science, Volume 5, Issue Supplement_S1, December 2021, Pages S1–S5, https://doi.org/10.1093/tas/txab171
Whole Genome Sequencing and Variant Filtering DNA collected from the affected calf, the dam, the sire, and three paternal half-siblings was sent to Admera Health (South Plainfield, NJ) for KAPA library prep and 150 bp paired-end sequencing on an Illumina NovaSeq to a targeted sequencing depth of 12X
Estandia-Ortega, B.; Reyna-Fabián, M.E.; Velázquez-Aragón, J.A.; González-del Angel, A.; Fernández-Hernández, L.; Alcántara-Ortigoza, M.A. The Enigmatic Etiology of Oculo-Auriculo-Vertebral Spectrum (OAVS): An Exploratory Gene Variant Interaction Approach in Candidate Genes. Life 2022, 12, 1723. https://doi.org/10.3390/life12111723
Libraries were sequenced on an Illumina MiSeq 2 × 150 platform (San Diego, CA, USA) through the Admera Health Company (South Plainfield, NJ, USA).
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Purified libraries from each mouse were pooled at equal ratios based on lung weight to ensure even sequencing depth per cell, purified once more with Sera-Mag Select beads to remove excess free primers, and sequenced on the Illumina HiSeq 2500 or NextSeq 550 platform (Admera Health Biopharma Services).
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The authors thank the patients who contributed specimens to this study; the Northwestern Skin Disease Research Center (DNA/RNA Delivery Core), the Robert H. Lurie Comprehensive Cancer Center (Pathology Core), the Center for Cancer Genome Discovery, the Yale Center for Genome Analysis, and Admera Health for their sequencing support;
Daniels J, Doukas PG, Escala MEM, Ringbloom KG, Shih DJH, Yang J, Tegtmeyer K, Park J, Thomas JJ, Selli ME, Altunbulakli C, Gowthaman R, Mo SH, Jothishankar B, Pease DR, Pro B, Abdulla FR, Shea C, Sahni N, Gru AA, Pierce BG, Louissaint A Jr, Guitart J, Choi J. Cellular origins and genetic landscape of cutaneous gamma delta T cell lymphomas. Nat Commun. 2020 Apr 14;11(1):1806. doi: 10.1038/s41467-020-15572-7. PMID: 32286303; PMCID: PMC7156460.
We thank Admera Health for performing next generation sequencing
Chung AS, Mettlen M, Ganguly D, Lu T, Wang T, Brekken RA, Hsiehchen D, Zhu H. Immune Checkpoint Inhibition is Safe and Effective for Liver Cancer Prevention in a Mouse Model of Hepatocellular Carcinoma. Cancer Prev Res (Phila). 2020 Nov;13(11):911-922. doi: 10.1158/1940-6207.CAPR-20-0200. Epub 2020 Aug 24. PMID: 32839204; PMCID: PMC8284815.
We would like to thank... and Admera Health (Yun Zhao) for genomics
Maggio AG, Shu HT, Laufer BI, Bi C, Lai Y, LaSalle JM, Hu VW. Elevated exposures to persistent endocrine disrupting compounds impact the sperm methylome in regions associated with autism spectrum disorder. Front Genet. 2022 Aug 11;13:929471. doi: 10.3389/fgene.2022.929471. PMID: 36035158; PMCID: PMC9403863.
The sperm DNA samples were sent to Admera Health (South Plainfield, NJ, United States), a CLIA-certified laboratory, for WGBS analyses.
