Ultra-high-resolution genomics for the most complete human, animal, plant, and microbial genomes
PacBio Whole Genome Sequencing
Long-Read WGS
PacBio High Fidelity (HiFi) Sequencing utilizes Single Molecule, Real-Time (SMRT) technology to deliver long reads (10-25kb) with unparalleled accuracy ≥99.9%, or Q30). HiFi sequencing provides the most complete and accurate view of the genome, transcriptome, and epigenome, taking research to the next level.
Unlock complete, accurate, and highly contiguous genomes with PacBio Revio long-read sequencing. Designed for complex genomes and challenging samples, our Revio WGS services deliver the clarity and confidence researchers need for discovery, assembly, and variant analysis.
Applications of Long-Read WGS
Resolve Structural Variants with Confidence
Resolve large insertions, deletions, inversions, and translocations ideal for discovering disease-driving SV’s
Complete, Phased Genome Assembly
Full haplotype phasing generates chromosome-scale assemblies. Bridge repetitive elements and duplications for highly contiguous, reference-quality genomes
Hybrid or Reference-Free Genome Assembly
Generate de novo genomes for non-model organisms with exceptional continuity and accuracy
PacBio WGS Workflow
Every WGS project is handled with meticulous attention to detail. Our workflow includes rigorous quality control at the sample, library, sequencing, and data levels, ensuring accurate, high-quality results. From genomics DNA preparation to variant discovery, we prioritize reliability and reproducibility to deliver biologically meaningful insights for your research.
Genomic DNA QC & Shearing
HMW DNA is extracted & QC’ed
Input: ≥1 µg gDNA per SMRT Cell 8M
DNA is sheared to ~15–20 kb for WGS
Library Construction
Libraries are prepared and indexed for multiplexed sequencing. Library prep is optimized for coverage & accuracy
Sequencing on the PacBio Revio
Libraries are sequenced for high-fidelity (HiFi), long-read WGS data
Data Analysis: SMRT Link
Comprehensive bioinformatics: genome assembly, variant detection, structural variation
Frequently Asked Questions
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For PacBio WGS, we accept human, animal, plant, bacteria, and fungi.
Human, animal, & plant samples: We recommend 15 ug and >100ng/uL concentration.
Bacteria & fungi samples: We recommend 8 ug and 100ng/uL concentration.
If you have questions about your sample type, please contact us. -
We provide comprehensive downstream analysis and interpretation including:
Variant DiscoveryQC + Mapping + Duplicate Detection + Variant Detection + annotation (if available) + Structural Variation + insertion site analysis
Assembly for small genomes (bacterial & yeast)
genome fasta files + reference-based gene annotation + online genome browser
Assembly for large genomes (human, hamster, CHO, etc)
genome fasta files + reference-based gene annotation + online genome browser + haplotype sequences for mammalian genomes
If you have further questions, please contact us to get in touch with a specialist.
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Our typical turnaround time for PacBio WGS projects is 4-6 weeks. Please connect with us if you require expedited services, we are happy to create a timeline that works best for your project goals.
More PacBio Long-Read Services
Full-length 16s Amplicon-seq
With PacBio Revio, full-length transcript sequencing becomes seamless. HiFi reads span entire isoforms end to end, eliminating the need for assembly and enabling confident detection of alternative splicing, fusion events, and novel transcripts. This level of clarity supports functional annotation, biomarker discovery, and both bulk and single-cell transcriptomics.
Full-Length RNA-seq (Iso-seq)
Revio delivers highly accurate long reads that resolve complex genomic regions and structural variants that short-read platforms often miss. HiFi WGS supports de novo assembly, haplotype phasing, variant calling, and comprehensive analysis across human, model organism, and microbial genomes.
Save on Long-Reads with Revio
Access savings on long-read savings on RNA, DNA, and premade library sequencing when you run on the Revio.