Metagenome Taxonomy Kraken2 Demo Report
Additional Analysis Figures
Publications
The V3-V4 region of the 16 S rRNA gene was targeted to obtain the fragment libraries, which were amplified as described by the Illumina protocol and with primers of Klindworth et al. [19] on an Illumina MiSeq sequencer with 2 × 300 bp chemistry by a provider known as Admera Health (South Plainfield, NJ, USA).
Expanding Antarctic biogeography: microbial ecology of Antarctic island soils
sent to Admera Health Biopharma Services (USA) for Illumina Hiseq whole genome shotgun sequencing.
The WGA product was processed through a Nextera XT library and sequenced on an Illumina HiSeq X machine by Admera Health (South Plainfield, NJ, USA), producing 37,100,964 pairs of 150 bp reads
Libraries were pooled and submitted to Admera Health (South Plainfield, NJ, United States)
Therefore, the DNA material obtained from WGA was processed with a Nextera XT library and sequenced at Admera Health (South Plainfield, USA), using Illumina HiSeq X technology to generate 75,709,674 and 74,962,776 reads (paired-ends 2 × 150 bp) for IPSal+sm and IPSal+b populations, respectively.
The libraries were sequenced at Admera Health, LLC (NJ, USA) using an Illumina HiSeq X sequencer (150-bp paired-end reads). Bioinformatics analysis was conducted using KBase.
Metatranscriptomics Analysis of Ileum Microbial Communities in Necrotic Enteritis Chickens
The library preparation and sequencing process was conducted at Genohub (Admera Health Biopharma Services) using the Universal Prokaryotic RNA-Seq Library Preparation Kit (Tecan, California, USA) for the library preparation and Illumina HiSeq system for the sequencing of total RNA
Finally, faecal shotgun metagenomics libraries of 3 biological replicates of 24-month-old AL, DR, AL_DR16M and AL_DR20M, and negative controls, were sequenced at 250x2 pairedend sequencing on an Illumina HiSeq 2500 at Admera Health, USA.
The sequencing was done on an Illumina MiSeq v2 platform by Admera Health, with 2x250bp paired end reads and a sequencing depth at 24-30M reads.
Libraries for sequencing were prepared using the Nextera XT library Prep Kit (Illumina) and sequenced on an HiSeq X in an external facility (Admera Health LLC, NJ, USA) in paired end, with 150 bp read long.
Risk Factors for Antimicrobial Resistance in Turkey Farms: A Cross-Sectional Study in Three European Countries
The samples were sequenced on the NovaSeq 6000 platform (Illumina Inc, CA, USA) by Admera Health (South Plainfield, NJ, USA), using a 2 × 150-bp paired-end (PE) read approach, aiming for 35 M PE reads per sample.
The shotgun sequencing was carried out by a commercial sequencing service provider (Admera Health, South Plainfield, NJ, USA).
were sequenced on an Illumina HiSeq 4000 (2 × 150 cycles, paired end) at Admera Health, New Jersey (USA).
Library preparation and sequencing of the Illumina data were conducted through Genohub (Austin, TX) and Admera Health (South Plainfield, NJ).
DNA was shipped for sequencing to an external provider (Admera Health, NJ, USA)
The construction of the library was carried out commercially (ADMERA HEALTH LLC, USA).
The FloraPrep™ sample collection tubes and primers were from Suzhou Admera Health Medical Technology Co., Ltd.
Metagenomes were sequenced using Illumina Nextseq (150 bp, paired-end reads) (Admera Health, NJ, USA).
Analysis for total bacteria, fungi and protist was performed via multiplex amplicon sequencing in a HiSeq 2500 System® - Rapid Mode 2 × 250 bp paired-end (Illumina Inc., San Diego, USA) in Admera Health (Admera Health, New Jersey, US).
The remaining samples (i.e., 56 samples) associated with long-term storage (4, 8, and 12 months) of P1 and S1 were sequenced on an Illumina HiSeq 4000 (2 × 150 cycles, paired end) at Admera Health, New Jersey (USA).
Libraries were sequenced using Illumina MiSeq v3 technology (2 × 300 bp, paired-end) by Admera Health (San Francisco, CA, USA) with 30% PhiX DNA as spike-in.
The construction of the library was done commercially (ADMERA HEALTH LLC, USA).
Fecal DNA samples were sequenced using the Illumina NovaSeq apparatus by Admera Health (USA).
Degradation profile of nixtamalized maize pericarp by the action of the microbial consortium PM-06
The sequencing of the extracted mgDNA (8 h samples), was carried out at Admera Health Inc. (NJ, USA), using the Illumina NextSeq platform.
The pooled PCR products were sequenced at an Illumina HiSeq 2X250bp at Admera Health (New Jersey, USA)
Samples were shipped on icepacks to Admera Health (South Plainfield, NJ) for DNA extraction and 16S rRNA sequencing using the Floracheck™ (Admera Health) Mammalian assay to determine the V3 and V4 hypervariable regions.
Up to 15 ng of isolated genomic DNA was used to amplify via PCR with proprietary primers (Admera Health) covering hypervariable regions V3 and V4
For library preparation, 50 ng of isolated genomic DNA were used and amplified via PCR with proprietary primers (Admera Health, NJ, USA) covering hypervariable regions V3 and V4.
Potential role of rumen bacteria in modulating milk production and composition of admixed dairy cows
Library preparation and sequencing were performed at Admera Health Limited (USA).
MiDAS 5: Global diversity of bacteria and archaea in anaerobic digesters
The four clonal libraries were sent to Admera Health (Plainfield, NJ, USA) for PacBio library preparation and sequencing